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. 2021 Dec 29;3(1):101070. doi: 10.1016/j.xpro.2021.101070

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Proliferative capacity of isolated early erythroid progenitor populations

(A) Ex vivo growth curves of FACS-isolated CD71/CD24^low (n = 4) and CD71/CD24^high (n = 3) cells, and magnetically isolated CD71/CD24^low cells (n = 4) grown in Dex-free erythroid expansion media. Comparing points of maximum proliferation, FACS CD71/CD24^low vs Magnet CD71/CD24^low p = 0.860; FACS CD71/CD24^low vs FACS CD71/CD24^high p < 0.001; Magnet CD71/CD24^low vs FACS CD71/CD24^high p < 0.001.

(B) Ex vivo growth curve of FACS-isolated and magnetically isolated CD71/CD24^low cells (both n = 4) grown in media supplemented with and without 100 nM Dex. Comparing points of maximum proliferation, FACS CD71/CD24^low (no Dex) vs FACS CD71/CD24^low (+ Dex) p < 0.001; Magnet CD71/CD24^low (no Dex) vs Magnet CD71/CD24^low (+ Dex) p < 0.001.

(C) Ex vivo growth curve of FACS-isolated CD71/CD24^high cells (both n = 3) grown in media supplemented with and without 100 nM Dex. Comparing points of maximum proliferation, FACS CD71/CD24^high (no Dex) vs FACS CD71/CD24^high (+ Dex) p = 0.813. All error bars represent standard error of the mean.