Table 2.

Average number of cells counted from three technical replicates, scaled averages, and average expansion per single cell on days 2 and 6 after initial seeding

Day after initial seeding	Media supplement	Average number of cells	Scaled average number of cells	Average expansion per single cell
2	No Dex	527,600	527,600	53
2	Dex	600,317	600,317	60
6	No Dex	212,733	7,764,767	776
6	Dex	469,150	30,003,081	3000

Note: From the raw cell count data (Table 1), the average number of cells for each biological replicate was generated (Table 2). To generate scaled averages, which accounts for re-seeding cells at 50,000 cells per well on days 3 and 6 after initial seeding, cell count averages on days 6, 8, and 10 were divided by the number of cells re-seeded (50,000 cells) and multiplied by the most recent scaled average value before re-seeding (Table 2). Average expansion per single cell was calculated by dividing each scaled average by the number of cells seeded on day 0 (10,000 cells).