PNEG buffer | Final concentration | Amount |
---|---|---|
1× phosphate buffered saline (PBS) | n/a | 500 mL |
0.5 M EDTA | 20 μM | 200 μL |
Newborn calf serum | 2% | 10 mL |
40% w/v glucose stock solution in water | 10 mM | 2 mL |
Total | n/a | 500 mL |
PNEG buffer | Final concentration | Amount |
---|---|---|
1× phosphate buffered saline (PBS) | n/a | 500 mL |
0.5 M EDTA | 20 μM | 200 μL |
Newborn calf serum | 2% | 10 mL |
40% w/v glucose stock solution in water | 10 mM | 2 mL |
Total | n/a | 500 mL |