Serum-free erythroid liquid expansion media (± 100 nM Dex)	Final concentration	Amount
Stemspan SFEM II	n/a	50 mL
Recombinant murine SCF (stock conc. 100 mcg/mL)	100 ng/mL	50 μL
Recombinant murine IGF-1 (stock conc. 80 mcg/mL)	40 ng/mL	25 μL
Recombinant human Epo (stock conc. 20,000 U/mL)	2 U/mL	5 μL
Penicillin/Streptomycin (100×)	1%	500 μL
Dexamethasone (Dex) (1 mM in methanol)	100 nM	5 μL
Total	n/a	50 mL