Stratification of observed peptides by HLA allele using NetMHCpan binding prediction.A, proportion of all peptides predicted to bind (rank score < 0.5) to concomitant HLA molecules by NetMHCpan 4.1. B, proportion of predicted binders exclusively identified by each search engine. C–E, panels show from left to right the sequence logo for peptide 9-mer motif, total number (n), and proportion (n/total) of peptide sequences predicted to bind (rank < 0.5) to concomitant HLA molecules by NetMHCpan 4.1 for each immunopeptidomic dataset investigated.