Figure 3.

Behavior evaluation of mice receiving intracranial injection of synthetic Luc-mRNA

Four groups of 8-week-old C57 mice were intracranially injected with synthetic Luc-mRNA in TransIT-mRNA solution or TransIT-mRNA reagent alone. The behavior tests were performed 14 days after injection. (A–E) Y maze test. (A) Infrared video recorder monitored the activity trajectory of mice in Y maze within 8 min. The overall traveled distances in the Y maze were recorded. (B) Total distances of the mice traveled in the Y maze. (C) Alternation triplet times of the mice traveling in the Y maze. (D) Total times of the mice traveling in the center of the Y maze. (E) The percentage of alternation triplet of the mice traveled in Y maze. (F–J) Open field test. (F) Infrared video recorder recorded the activity trajectory of mice in open field within 10 min. (G) Total distance the mice traveled in the open field. (H) Total times of the mice traveled in the center of open field. (I) Total distance of the mice traveled in the center of open field. (J) Total times of the mice entering the center of the open field.