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. 2021 Dec 14;24:160–170. doi: 10.1016/j.omto.2021.12.010

Figure 5.

Figure 5

The effects of synthetic TRAIL-mRNA on DBTRG-Luc glioma cells

(A and B) Flow cytometric analysis (A) and statistic (B) of synthetic TRAIL-mRNA induced apoptosis in DBTRG-Luc cells using annexin V-FITC/PI. (C) Cell viability determined by RTCA at an interval of 5 min until the end of 72 h. (D) After the experiment, the cells remaining in the E-plate culture plate were observed under the microscope at 400×. (E and F) The expression levels (A) and statistic (B) of apoptosis-related proteins in DBTRG-Luc cells determined by western blot at 24 h after TRAIL-mRNA transfection. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.