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. 2022 Jan 1;78(Pt 1):25–30. doi: 10.1107/S2053230X21012632

Table 2. Crystallization.

Method Vapor diffusion, sitting drop
Plate type 96-well, Compact 300, Rigaku
Temperature (K) 287
Protein concentration PxSDR (BuxeA.00010.c.B1.PS02595) at 22.5 mg ml−1
Buffer composition of protein solution 25 mM HEPES pH 7.0, 500 mM NaCl, 5% glycerol, 2 mM DTT, 0.025% azide
Composition of reservoir solution Rigaku Reagents JCSG+ screen H11: 0.2 M magnesium chloride, 0.1 M bis-Tris pH 5.5, 25% PEG 3350
Volume and ratio of drop 0.4 µl protein:0.4 µl reservoir (1:1)
Volume of reservoir (µl) 80