Table 2. Crystallization.
Method | Vapor diffusion, sitting drop |
Plate type | 96-well, Compact 300, Rigaku |
Temperature (K) | 287 |
Protein concentration | PxSDR (BuxeA.00010.c.B1.PS02595) at 22.5 mg ml−1 |
Buffer composition of protein solution | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% glycerol, 2 mM DTT, 0.025% azide |
Composition of reservoir solution | Rigaku Reagents JCSG+ screen H11: 0.2 M magnesium chloride, 0.1 M bis-Tris pH 5.5, 25% PEG 3350 |
Volume and ratio of drop | 0.4 µl protein:0.4 µl reservoir (1:1) |
Volume of reservoir (µl) | 80 |