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. 2022 Jan 1;78(Pt 1):31–38. doi: 10.1107/S2053230X21013078

Table 3. Crystallization of FolM alternative dihydrofolate reductase 1 from B. suis (BsFolM).

Method Vapor diffusion, sitting drop
Plate type Rigaku Reagents XJR
Temperature (K) 290
Crystallization BsFolM (19 mg ml−1) incubated with 4 mM NADPH, mixed 1:1 with MCSG1 condition A1 [20%(w/v) PEG 8000, 100 mM HEPES pH 7.5]
Composition of reservoir solution 20%(w/v) PEG 8000, 100 mM HEPES pH 7.5
Volume and ratio of drop 0.4 µl:0.4 µl
Volume of reservoir (µl) 80