Table 3. Crystallization of FolM alternative dihydrofolate reductase 1 from B. suis (BsFolM).
Method | Vapor diffusion, sitting drop |
Plate type | Rigaku Reagents XJR |
Temperature (K) | 290 |
Crystallization | BsFolM (19 mg ml−1) incubated with 4 mM NADPH, mixed 1:1 with MCSG1 condition A1 [20%(w/v) PEG 8000, 100 mM HEPES pH 7.5] |
Composition of reservoir solution | 20%(w/v) PEG 8000, 100 mM HEPES pH 7.5 |
Volume and ratio of drop | 0.4 µl:0.4 µl |
Volume of reservoir (µl) | 80 |