Table 1. Macromolecule-production information.

Source organism	Micromonospora chersina
DNA source	E. coli codon-optimized synthesis (gBlock) from Genewiz
Insert forward primer†	5′-agaacctgtacttccaatccATGTCGACTAAATCTGTATT-3′
Insert reverse primer	5′-ACCAGACCAGATGTGAacggtctccagtaaaggtgg-3′
Vector forward primer	5′-TTAAACCAGACCAGATGTGAacggtctccagtaaaggtg-3′
Vector reverse primer	5′-agaacctgtacttccaatccATGTCGACTAAATCTGTATT-3′
Expression vector‡	pNIC28-Bsa4
Expression host	Escherichia coli BL21 (DE3)
Complete amino-acid sequence of the construct produced§	mgsshhhhhhenlyfq/sMSTKSVLFGRPVQTEGVPNVYAGAPVVPWTPPEPGIDNLGINSIDTFAVPGVGEYTVAFDGWVRVVRSPSTSGEWADAEVYTNLIEMKMVGECEELGKITVTLNPDCLSAGQIRTPFDPYAGEGPSAKACRMAVGAIFDMPKLGLKLMNREPIILTIDDVRSIPPAGAPGKGQIYRMMPLLDVNDPDGQPVAYLTSLRFNMGGYLKPDQM

^†The primers contained overhangs for cloning, which are represented as lower case letters.

^‡Plasmid pNIC28-Bsa4 was a gift from Opher Gileadi (Addgene plasmid #26103).

^§The expression and purification tag is shown in lower case with the TEV recognition site underlined and the cut site indicated with a slash.