Table 2. Crystallization.

Method	Vapor diffusion, sitting drop
Plate type	MRC 2 (96-well crystallization plate)
Temperature (K)	293
Protein concentration (mg ml−1)	20
Buffer composition of protein solution (crystallization buffer)	0.025 M Tris pH 8.0, 0.15 M NaCl, 1 mM DTT
Composition of reservoir solution	25%(w/v) PEG 1500, 0.1 M PCB buffer pH 7.0
Volume and ratio of drop†	300 nl (1.5:1)
Volume of reservoir (µl)	50

^†The initial screening for crystals was performed using a 1:1 ratio of protein to reservoir solution.