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. 2022 Jan 1;78(Pt 1):8–16. doi: 10.1107/S2053230X21012413

Figure 3.

Figure 3

Schematic of the flash-freezng process for large crystals and its key points. (a) Material setup prior to flash-freezing. Care should be taken in choosing an appropriate cryoloop diameter and in ensuring that both the crystal and a large portion of the cryoloop stem are well coated in cryoprotectant. In preparation for freezing, the crystal-containing cryoloop is mounted on the goniometer while an object (card) blocks the cryostream. (b) After mounting, quick removal of the card leads to a vitreous flash-freeze with the absence of crystalline ice.