FIG. 3.

The expression of ARF6(T27N) can be tightly controlled by AdCre. 3T3-L1 preadipocytes were stably infected with a version of the inducible retrovirus designed to encode either GFP or ARF6(T27N) following AdCre infection. Adipocytes from these GFP and ARF6(T27N) lines were then either mock infected (−) or infected with AdCre (+). Total cellular lysates were prepared 6 days later and subjected to SDS-PAGE and Western immunoblotting with the antisera indicated. Even following extended exposure, virtually no HA-tagged ARF6(T27N) could be detected prior to infection with AdCre. Sizes are indicated in kilodaltons.