FIG. 4.
Expression of a dominant negative mutant of ARF6 in 3T3-L1 adipocytes does not affect insulin-stimulated hexose uptake. Six days following induction of GFP (a and b) or ARF6(T27N) (c and d) expression by infection with AdCre, 3T3-L1 adipocytes were treated with the indicated concentration of insulin for 20 min or left in medium without hormone, and the uptake of 2-[3H]deoxyglucose was measured. Nonspecific uptake measured in the presence cytochalasin B in the absence of insulin was subtracted from all values. The results shown are the means ± SEM of four independent experiments, each performed in triplicate.