FIG. 6.

Expression of a dominant negative mutant of ARF6 in 3T3-L1 adipocytes inhibits the ability of activated Gαq to stimulate hexose uptake. Three days following induction of GFP (a) or ARF6(T27N) (b) expression by AdCre, adipocytes were either mock infected (Basal) or infected with a recombinant adenovirus expressing Gαq(Q209L) (multiplicity of infection of ∼20). The uptake of 2-[³H]deoxyglucose was measured 72 h later. The results shown are the means ± SEM of four independent experiments, each performed in duplicate. The asterisk denotes the statistically significant difference (P = 0.003) for Gαq-stimulated ARF6(T27N) cells not infected compared to infected with AdCre and that (P = 0.003) for Gαq-stimulated AdCre-infected ARF6(T27N) cells compared to similarly treated GFP cells.