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. Author manuscript; available in PMC: 2022 Jan 4.
Published in final edited form as: ACS Appl Mater Interfaces. 2019 May 13;11(20):18233–18241. doi: 10.1021/acsami.9b04362

Figure 1.

Figure 1.

Fabrication of the reference-free TFM platform. (a) Fluorophore-labeled PEG used to fabricate fiducial markers is synthesized by coupling a diamine-PEG to either an acrylate and a fluorophore or two acrylates using NHS esters. (b,c) Resulting acrylated-PEG species are photocoupled into a preformed hydrogel by soaking the hydrogel in a solution containing the PEG species, a radical generating photoinitiator (LAP), and a comonomer (NVP). (d,e) Resulting fiducial markers are patterned in large arrays within the upper ~20 μm of the base hydrogel.