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. 2022 Jan 4;24:1. doi: 10.1186/s13058-021-01497-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — circCCDC85A acts as a miR-550a-5p sponge in breast cancer cells. A The potential miRNA targets of circCCDC85A were predicted by CircNet online database, and it was found that circCCDC85A may function as the sponge of miR-660-3p, miR-550a-5p and miR-4508. B The potential downstream pathways of the above three miRNAs were analyzed through tarbase database, and miR-550a-5p was found to be associated with the Hippo signaling pathway which is a well-known cancer-related pathway C FISH results showed that both circCCDC85A and miR-550a-5p were predominantly located in cytoplasm. D The miranda database was applied for prediction of the binding sites between circCCDC85A and miR-550a-5p, and it was found that there are two potential binding sites between hsa-miR-550a-5p and hsa_circ_0120472. E Immunoprecipitation of Myc-tagged AGO2 from MCF-7 cells transfected with either Myc-AGO2 or Myc-vector negative control plus miR-550a-5p mimics. The enrichment of circCCDC85A was detected by qRT-PCR. F Luciferase reporter assay was performed to detect the luciferase activity of luc-circCCDC85A or luc-vector negative control in MCF-7 cells co-transfected with miR-550a-5p mimics. *P < 0.05