Fig. 6.

circCCDC85A restores miR-550a-5p mediated targeting repression of tumor suppressor MOB1A in breast cancer cells. A DIANA TOOLS was performed to predict the downstream signaling pathway of miR-550a-5p. B Targetscan, miRWalk and miRDB database were applied for prediction of the potential target genes of miR-550a-5p, and Venn diagram presented the intersection element of “Targetscan”, “miRWalk”, "miRDB" and "Hippo pathway". C The wild type 3’UTR and mutant 3’UTR of MOB1A were constructed into the human Renilla luciferase reporter vector. D Luciferase reporter assay was performed to detect the luciferase activity of wild type MOB1A 3’UTR vector or mutant MOB1A 3’UTR vector in MDA-MB-231 cells co-transfected with miR-550a-5p mimics or negative control vector. E MOB1A mRNA expression in MDA-MB-231 cells transfected with miR-550a-5p, circCCDC85A or miR-550a-5p plus circCCDC85A was detected by qRT-PCR. F MOB1A protein expression in MDA-MB-231 cells transfected with miR-550a-5p, circCCDC85A or miR-550a-5p plus circCCDC85A was detected by western blot. *P < 0.05, **P < 0.01, ***P < 0.001