Fig. 11.

Engineered sequential pH/redox responsive nanoparticles (PC7A-ss-DOX) for precisely delivering chemodrug (DOX) and immunoadjuvant (imidazoquinolines, IMDQs) for synergistic chemo-immunotherapy. a Schematic diagram of the chemical structure of PC7A-ss-DOX and the sequential immune activation mechanism by PC7A-ss-DOX. b, c TEM images of PC7A-ss-DOX at different pH values. Scale bar, 100 nm. d In vitro DOX release from PC7A-ss-DOX under different conditions. e Immunofluorescence images of CRT after various treatments. Scale bar, 20 μm. f ATP levels in tumor cells after different treatments. g The content of HMGB1 in the tumor cells treated by different conditions and schematic diagram of DC maturation under TLR priming. h RAW-Blue reporter cells number after being stimulated by different materials. i Percentage of mature DCs in different groups: (1) B16-OVA; (2) PC7A-ss-DOX treated B16-OVA; (3) B16-OVA + iPDPA-IMDQ; (4) PC7A-ss-DOX treated B16-OVA + iPDPA-IMDQ. The tumor growth curves of j primary tumors upon different treatments and corresponding k distant tumors of mice