FIG. 3.
NFκ-B activation leads to FLIP protein and mRNA upregulation. (A) The HT1080 fibrosarcoma cell line (wt or I-κBmut), stably transfected with a myc-tagged, mutated, nondegradable version of I-κBα, were treated for the indicated periods of time with TNF at 50 ng/ml and analyzed for FLIPL, FLIPS, and TRAF1 expression by immunoblot assay. (B) RNase protection assay analysis of mRNA levels of various apoptosis-related genes in wt or I-κBmut HT1080 cells after treatment with TNF as described above, using different hApo multiprobe template sets. L32 and glyceraldehyde-3-phosphate dehydrogenase (GADPH) were included in each template set as internal controls.