FIG. 5.
NFκ-B activation leads to FLIP upregulation in Jurkat T cells. (A) Both wt and NEMO (N.)-deficient (def.) Jurkat cells were stimulated for the indicated periods of time with PMA and ionomycin (Iono) (or phosphate-buffered saline [control]) and analyzed for FLIPL, FLIPS, and phosphorylated IκB expression by immunoblot assay. (B) Cells were stimulated with TNF at 100 ng/ml, and FLIPL expression was monitored as in panel A. Expression of NEMO is demonstrated on the right. (C) Cell death induced by TNF in wt and NEMO-deficient cells, respectively.