FIG. 7.

TRAF1, TRAF2, or cIAP1 overexpression fails to protect HT1080 cells from TNF-, FasL-, and TRAIL-induced cell death. (A) pBape-Flag-tagged TRAF1 and TRAF-2 vectors were introduced by virus infection into HT1080 wt or I-κBmut cells. TRAF1 and TRAF2 expression was determined by Western blot assay using anti-TRAF1, anti-Flag, or anti-TRAF2 antibodies. The sensitivity to TNF-mediated cell death of the cell pools obtained was determined essentially as described in the legend to Fig 5. (B) HT1080 wt or I-κBmut cells were infected with either pBape-myc-tagged cIAP1 or empty vector. Cellular pools were analyzed for resistance to TNF as described above. The values beside the blots are molecular sizes in kilodaltons.