Table 6.

Ames test results of Py-mulin using S. typhimurium strains TA97, TA98, TA100, TA102 and TA1535

Substance	Dose (μg/plate)	TA97		TA98		TA100		TA1535
		- S9	+ S9	- S9	+ S9	- S9	+ S9	- S9	+ S9
Py-mulin	1000	46.3 ± 3.5**	55.0 ± 4.6**	21.0 ± 2.6	19.7 ± 3.5	36.7 ± 3.1**	38.0 ± 3.6**	20.7 ± 3.1	27.3 ± 2.5
	200	62.0 ± 5.3	62.7 ± 4.2**	22.7 ± 3.1	26.7 ± 2.5	83.7 ± 4.5*	73.3 ± 3.1**	25.0 ± 2.6	38.7 ± 3.5
	40	72.0 ± 3.6	77.7 ± 3.2	33.3 ± 4.5	28.0 ± 4.9	93.7 ± 4.2	89.0 ± 8.5	34.3 ± 4.0	41.7 ± 4.7
	8	80.3 ± 4.7	79.3 ± 5.1	23.7 ± 4.0	24.7 ± 5.5	105.7 ± 6.7	101.3 ± 3.5	38.7 ± 4.7	36.7 ± 5.0
	1.6	76.3 ± 5.6	94.3 ± 4.0	32.7 ± 3.5	31.7 ± 3.1	112.0 ± 5.3	115.0 ± 5.0	42.0 ± 4.4	44.3 ± 5.5
Vehicle control		84.0 ± 5.0	98.3 ± 5.1	24.7 ± 3.5	29.7 ± 2.1	111.3 ± 4.2	120.0 ± 5.6	34.7 ± 4.2	43.3 ± 5.7
Positive control		737.3 ± 13.3**	964.7 ± 13.0**	567.7 ± 11.5**	614.0 ± 15.1**	913.3 ± 20.8**	1212.3 ± 12.9**	583.0 ± 13.7**	746.3 ± 12.6**

Note: The numbers indicate the means and standards deviation values of CFU in triplicate assay systems

+ S9: with metabolic activation; − S9: without metabolic activation

Vehicle contro: DMSO (100 μL/plate)

Positive control: 2-aminofluorene for all strains with S9; Fenaminosulf for all strains without S9

*p < 0.05, **p < 0.01 vs. vehicle control