Fig. 2.

High percentages of RORγt⁺ cells during the first few days of life. a. Representative pseudocolor dot plots of total RORγt⁺ cells, Th17 and ILC3. b–d Dynamic changes of total RORγt⁺ cells, Th17 and ILC3. Th17 was gated on CD3e⁺CD4⁺CD8a⁻. ILC3 was gated on CD3e⁻CD8a⁻B220⁻LIN⁻CD90.2⁺. LIN includes CD11b and CD11c. Three independent experiments were performed, n = 4–5 per group. Data were shown as mean values ± SD. Statistical analyses were performed with Student’s two-tailed unpaired t-test. *Compared with day 1. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001