Fig. 3.

IL-17A⁺ RORγt⁺ cells increase in intestine of NEC mice. a. Diagram of the method to induce NEC. b, c Flow cytometric quantification of total RORγt⁺ cells and related cytokines (IL-17A and IL-22) in the lamina propria of mice with or without NEC. d–g. Flow cytometric quantification of Th17 and ILC3, as well as IL-17A expressed of them. Three independent experiments were performed, n = 4–5 per group. Data were shown as mean values ± SD. Statistical analyses were performed with Student’s two-tailed unpaired t-test. *p < 0.05; **p < 0.01