FIG. 3.

Generation and analysis of Kif3C^null mutants in ES cells. (A) Strategy for generating Kif3C^null mutants. One 4.5-kb DNA fragment between BamHI and XhoI of the Kif3C gene was replaced with a 1.7-kb PGK-neo cassette. The targeting vector included a negative selection marker, PGK-tk. The targeting vector was linearized with XhoI and introduced into R1 ES cells. (B) Southern analysis of ES cells. ES cell genomic DNA was cut with XbaI and probed with a 1.5-kb BamHI/EcoRI DNA fragment. The wild-type (WT) and targeted Kif3C (Mut) alleles were detected as 10.0- and 7.5-kb bands, respectively. P1, P2, P3, and P4 indicate the locations of primers used for genotyping Kif3C^null mice (Fig. 4).