Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 3;22:1. doi: 10.1186/s12935-021-02402-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Expression analysis of SCUBE3 and establishment and validation of scube3 knockdown cell model. A SCUBE3 expression in tumour and normal tissues in the TCGA database from UALCAN database. SCUBE3 expression is based on the pa'tients sex, age, body weight, tumour grade and cancer stage. B, C RT-qPCR and western blot analysis of SCUBE3 mRNA and protein expression in normal hepatocytes HL7702 and four HCC cell lines (Bel7404, Bel7402, HepG2, and SMMC7721). D Western blotting was used to detect the expression level of SCUBE3 in the whole protein, cytoplasmic protein, and membrane protein samples of Bel7404 HCC cells. E Bel7404 cells were transfected with control shRNA (shCtrl) and three shSCUBE3 lentiviruses (shSCUBE3-1, shSCUBE3-2, shSCUBE3-3). Green fluorescence protein (GFP) expression illustrated to transfect with lentivirus successfully. F The expression levels of SCUBE3 in the cells were determined through reverse-transcriptase PCR. G–H The protein expression of SCUBE3 was examined by western blotting (n = 3, ns,no significance,*p < 0.05,**p < 0.01, and ***p < 0.001 compared with normal cells)