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. 2022 Jan 3;22:1. doi: 10.1186/s12935-021-02402-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Knockdown of SCUBE3 expression suppressed proliferation, promoted apoptosis, and induced cell cycle arrest in HCC cells. A MTT-assays were performed to evaluate the viability of negative control and cells expressing low levels of SCUBE3, and Colony formation assay using knockdown of SCUBE3 cells. B The proliferation rate of Bel7404 cells was reduced by SCUBE3 knockdown, which was observed using the Celigo cell counting assay. C EdU assays were used to determine the effect of SCUBE3 on the proliferation of Bel7404 cells. D Relative caspase activity measured by caspase 3/7 assay showed apoptosis in knockdown of SCUBE3 cells (shSCUBE3). E Effect of SCUBE3 on apoptosis was analyzed through flow cytometry using Annexin V-APC staining. F Cell cycle distribution after inhibition of SCUBE3 was analyzed through flow cytometry (n = 3,*p < 0.05, **p < 0.01, ***p < 0.001. ns, no significance, KD, knockdown, NC, negative control)