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. 2022 Jan 3;22:1. doi: 10.1186/s12935-021-02402-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — CCNE1 silence inhibit Bel7404 cell proliferation, migration, arrested G1/S transition and promote apoptosis. A 5 days of continuous MTT assays. B Knockdown of CCNE1 remarkably enhanced caspase-3/7 activity in the shCCNE1 group. C Knockdown of CCNE1 promoted cell apoptosis. D Analysis of cell cycle profiles after CCNE knockdown in Bel7404 cells. E Colony formation assay using cells infected with CCNE1 lentiviral vector and empty vectors. Representative images were acquired, and the colonies were counted after two weeks. F The inhibition effect of CCNE1 silence on cell migration was assessed by transwell assay. After 24 h of incubation, the cells were fixed and stained. Representative images of transwell chamber assays were acquired, and quantification of the number of cells which migrated through the basement membrane (n = 5 per group) (*p < 0.01, **p < 0.01,***p < 0.001 compared with shCtrl)