FIG. 4.

CBP bromodomain is involved in the interaction with acetylated MyoD. (A) Diagram of the CBP molecule, showing the C/H3 domain, bromodomain, and HAT domain. (B) Increasing doses (2, 10, and 50 ng) of recombinant MyoD were incubated with GST-CBP absorbed onto glutathione-coated beads in the presence or absence of acetyl-CoA; the presence of MyoD in association with CBP was detected by Western blotting. wt, wild type. (C to F) MyoD was incubated with wt CBP in the presence of acetyl-CoA (Ac-CoA) (or in the absence of Ac-CoA for the controls, as indicated), isolated from the enzyme, and used in a GST pull-down assay with beads coated with the indicated CBP mutants; the presence of MyoD in association with CBP was detected by Western blotting.