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Knockdown of LSD1 suppresses NRK-49F cells activation. The NRK-49F cells were transfected with Ad-shNC or Ad-shLSD1 and then treated with 10 ng/ml TGF-β1 for 72 h. A The expression and location of α-SMA were detected by IF assay (bar = 100 μm). B The protein levels of LSD1, α-SMA, Fibronectin, collagen I and collagen IV were evaluated by western blot. Each experiment repeated three times. **p < 0.01 vs. Control. ##p < 0.01 vs. TGF-β1 + Ad-shLSD1
