Fig. 5.

LSD1 activates TGF-β1/Smad3 pathway through suppressing SIRT3 expression. A The expression of SIRT3, TGF-β1 and Smad3 were determined by western blot in different rats groups. B The expression of SIRT3 and Smad3 were detected by western blot in different treated NRK-49F cells. The NRK-49F cells were transfected with Ad-LSD1 or SIRT3, or co-transfected with both Ad-LSD1 and SIRT3. C After cells pre-treated with TGF-β1, the LSD1, SIRT3 and Smad3 expression were evaluated by western blot. Each experiment repeated three times. *p < 0.05, **p < 0.01 vs. TGF-β1. ^#p < 0.05, ^##p < 0.01 vs. TGF-β1 + Ad-LSD1