Table 4.
Mode of inhibition of rat liver FAAH-1 oleamide hydrolysis by indomethacin and meclofenamate
| FAAH-1 Kinetics | Km (μM) | Vmax (nmol/min/mg protein) | Substrate |
|---|---|---|---|
| Control | 18.4 ± 3.5 | 4.6 ± 0.5 | Oleamide |
| + 200 μM indomethacin | 24.4 ± 3.3 | 3.8 ± 0.4 | Oleamide |
| + 100 μM meclofenamate | 22.7 ± 1.4 | 2.9 ± 0.1 | Oleamide |
| Control | 19.8 ± 2.0 | 8.4 ± 1.2 | Arachidonamide |
| + 200 μM indomethacin | 21.6 ± 2.8 | 7.2 ± 0.9 | Arachidonamide |
| + 100 μM meclofenamate | 23.4 ± 3.7 | 6.7 ± 1.0 | Arachidonamide |
Data are mean ± SEM of triplicate assessments conducted on five transient transfects (n = 5)