Fig. 4.

Expressions of mRNA for PGE₂ biosynthetic enzymes and EP receptor subtypes in colon after exposure to DSS. A Expression of mRNA for PGES and COX isozymes in colon from mice treated or not treated with the indicated dose of DSS for 7 days were analyzed by real-time RT-PCR (n = 7 to 12). Levels of mRNA expression are shown as the fold induction relative to the expression in WT mice without DSS administration (assigned the value “1”). *P < 0.05 vs WT mice within each day, ^†P < 0.05 vs non-DSS-treated WT mice, and ^‡P < 0.05 vs non-DSS-treated KO mice; 2-way ANOVA followed by Tukey multiple comparison test. B Expression of EP receptor mRNA in colon from mice treated with 1% DSS for indicated days was analyzed by real-time RT-PCR (n = 7 to 10). Levels of mRNA expression are shown as the fold induction relative to day 0 expression in WT (assigned the value “1”). *P < 0.05 vs WT mice within each day, ^†P < 0.05 vs WT at day 0, and ^‡P < 0.05 vs KO mice at day 0; 2-way ANOVA followed by Tukey multiple comparison test