Fig. 7.

Generation of IL-17A- and IFNγ-producing T cells in colonic LPMCs and splenocytes of mPGES-1^−/− mice with DSS-induced colitis. A Representative FCM plot of IL-17A-producing Th17 cells and IFNγ-producing Th1 cells in CD3⁺CD4⁺ T cells of splenocytes and colonic LPMCs isolated from WT and mPGES-1^−/− mice with colitis. Colonic LPMCs were pooled from 4 mice in each experiment on day 7 after the start of exposure to 1% DSS and analyzed by FCM, as described in the Methods (n = 5). B The ratio and the number of IL-17A⁺ and IFNγ⁺ cells in CD3⁺CD4⁺ T cells of colonic LPMCs on day 7 after the start of exposure to 1% DSS (n = 5). *P < 0.05 vs WT; t test