Fig. 8.

Effect of mPGES-1 gene deletion on the generation of Tregs in DSS-induced colitis. A Representative FCM plot of FoxP3⁺CD25⁺ Tregs in CD3⁺CD4⁺ T cells of colonic LPMCs, isolated from WT and mPGES-1^−/− mice with colitis. Colonic LPMCs were pooled from 4 mice in each experiment on day 7 after the start of exposure to 1% DSS and subjected to FCM analysis, as described in the Methods (n = 5). B The ratio and the number of FoxP3⁺CD25⁺ cells in CD3⁺CD4⁺ T cells of colonic LPMCs on day 7 after the start of exposure to 1% DSS (n = 5). C Expression of mRNA for IL-10 in the colon from mice treated or not treated with the indicated dose of DSS for 7 days was analyzed by real-time RT-PCR. Levels of mRNA expression are shown as the fold induction relative to the expression in WT without DSS administration (assigned the value “1”). *P < 0.05 vs WT within each day, ^†P < 0.05 vs non-DSS-treated WT mice, and ^‡P < 0.05 vs non-DSS-treated KO mice; 2-way ANOVA followed by Tukey multiple comparison test (n = 7 to 12)