Fig. 9.

Effect of CD4 positive T cell depletion on the exacerbated DSS-induced colitis in mPGES-1^−/− mice. A Schematic representation of the experimental plan. B The efficacy of in vivo CD4⁺ T cell depletion was confirmed by flow cytometry analysis of T cell population in the peripheral blood and spleen (n = 9 to 11). C Time course of change in total disease activity index (DAI) score of WT and mPGES-1^−/− mice after indicated days of exposure to 1% DSS (n = 9 to 11). On day 7 after the start of exposure to 1% DSS, the length of the colon (D) and weight of the spleen (E) were measured as indirect markers of inflammation (n = 9 to 11). F Expressions of mRNA for IL-17A, IFNγ, IL-10, IL-1β, IL-6, and TNFα in colon from mice treated with 1% DSS for 7 days were analyzed by real-time RT-PCR (n = 9 to 11). Levels of mRNA expression are shown as the fold induction relative to the expression in WT mice without DSS administration (assigned the value “1”). *P < 0.05; 2-way ANOVA followed by Tukey multiple comparison test