Effect of high DHA diet and three-week repetitive HDE exposure on BALF cytokine levels at three weeks and after the recovery period. Mice were fed DHA or control diets for four weeks before commencing three-week repetitive 12.5% HDE exposure. BALF was collected at the end of the three-week exposure, and BALF cytokine levels were determined as described in Methods. Left and right panels show data for three-week and recovery periods, respectively. Data are mean ± standard error of the mean. For IL-6(A), TNF-α (B), CXCL-1 (C) and AREG (D); n=6-8 for saline control and DHA alone groups (no HDE exposure) and n=11-13 mice for HDE and DHA+HDE groups. For MIF (E) and MPO (F); n=3 for control diet groups (saline control and HDE groups) and n=7 for DHA diet groups (DHA alone and DHA+HDE groups). * P<.05, ** P<.01, *** P<.001, ****P<.0001.