Fig. 6.

Changes in ARA and DHA-derived peripheral endocannabinoids after high DHA diet and three-week repetitive HDE exposure. Mice were fed DHA or control diets for four weeks before commencing three-week repetitive 12.5% HDE exposure. Plasma was isolated from whole bloods collected at the end of the three-week exposure and plasma levels of ARA-derived endocannabinoid AEA (A), non–endocannabinoid OEA (B), ARA-derived endocannabinoid 2-AG (C), DHA-derived endocannabinoid-like molecules DHEA (D) and 2DG (E) were determined by LC/MS as described in Methods. Data are mean ± standard error of the mean, n=5-8 for saline control and DHA alone groups (no HDE exposure) and n=12 mice for HDE and DHA+HDE groups except for 2DG (E) in the HDE alone group. * P<.05, ** P<.01, *** P<.001, **** P<.0001.