Fig. 7.

Effect of high DHA diet and repetitive HDE exposure on the number of Ym1-positive cells in the lung at three weeks (A-C) and after the recovery period (D-F). Mice were fed high DHA or control diets for four weeks before commencing three-week repetitive 12.5% HDE exposure. Lung was fixed in formalin, and paraffin embedded sections were obtained for immunofluorescence staining of Ym1 and images were obtained on Echo Revolve epifluorescence microscope with 20X objective as described in Methods. Lung representative images of Ym1+ immunofluorescence staining, number of Ym1+ macrophages and quantification of fluorescence intensity are shown on A, B and C for mice at three weeks after saline or HDE exposure, and on D, E and F for mice after the recovery period following 3-week repetitive HDE exposure. Number of Ym1+ cells were counted on ImageJ; n=5-7 for three weeks (B), and n=6 for the recovery period (E). Fluorescence intensity of the whole image was obtained by quantifying integrated density/area in Image J; n=4-6 for three weeks (C), and n=5-6 for the recovery period (F). Data are mean ± standard error of the mean. * P<.05, ** P<.01, *** P<.001, **** P<.0001.