Figure 2.

Upregulated MARK4 expression and pyroptosis in the P. gingivalis-infected BMDMs

(a) BMDMs induced by L929 conditional medium for 7 days or 10 days were identified by flow cytometry. Mature BMDMs were defined as CD11b⁺F4/80⁺ cultures. (b) BMDMs were infected with P. gingivalis (MOI = 10, 50 or 250) for 2 h, and gene transcription levels were assessed by real-time qPCR (n = 3). (c) Protein expression was analyzed by Western blot after bacterial infection for 24 h (MOI = 50) (n = 3). (d) IL-Iβ in the culture supernatant was detected by ELISA. *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control group.