Figure 3.

Reduced pyroptosis in P. gingivalis-infected BMDMs by MARK4 inhibition

(a) The cell viability was measured by the CCK8 assay. (b) BMDMs were pretreated with OTSSP167 (1 nM) or Compound 50 (1 μM) for 2 h and stimulated with P. gingivalis (MOI = 50) for 24 h, and the percentage of the death cell was measured by the LDH assay. (c) IL-Iβ and IL-18 in the culture supernatants of 24 h were detected by ELISA. (d) Gene transcription was assessed by real-time qPCR at 2 h. (e) Protein levels were analyzed by Western blot at 24 h. (f) The formation of ASC speck at 24 h were captured under a fluorescence microscope. White arrows indicated ASC specks. Scale bars, 50 µm. #p < 0.05; ##p < 0.01 compared with the control; *p < 0.05; **p < 0.01 compared with the P. gingivalis-treated group.