Table 1.
The sequences of primers
| Gene | Genbank ID | Forward Primer (5′ to 3′) | Reverse Primer (5′ to 3′) |
|---|---|---|---|
| mus-GSDMD | NM_026960 | TTCAGGCCCTACTGCCTTCT | GTTGACACATGAATAACGGGGTT |
| mus-Caspase-1 | NM_009807 | AATACAACCACTCGTACACGTC | AGCTCCAACCCTCGGAGAAA |
| mus-ASC | NM_023258 | CTTGTCAGGGGATGAACTCAAAA | GCCATACGACTCCAGATAGTAGC |
| mus-IL-1β | NM_008361 | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
| mus-IL-18 | NM_008360 | GACTCTTGCGTCAACTTCAAGG | CAGGCTGTCTTTTGTCAACGA |
| mus-MARK4 | NM_172279 | AGTGCAGTGGGCAGTGG | GGGGTGCTAGTGCTGTCC |
| mus-NLRP3 | NM_145827 | ATTACCCGCCCGAGAAAGG | CATGAGTGTGGCTAGATCCAAG |
| mus-β-actin | NM_007393 | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
| Caspase-1 | NM_033294 | TTTCCGCAAGGTTCGATTTTCA | GGCATCTGCGCTCTACCATC |
| ASC | NM_145182 | TGGATGCTCTGTACGGGAAG | CCAGGCTGGTGTGAAACTGAA |
| MARK4 | NM_031417 | TCACCCGCATCTCCAAC | CCCCTTCCCCTCCTGTA |
| NLRP3 | NM_001127462 | GATCTTCGCTGCGATCAACAG | CGTGCATTATCTGAACCCCAC |
| β-actin | NM_001101 | CTACCTCATGAAGATCCTCACCGA | TTCTCCTTAATGTCACGCACGATT |