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. 2021 Dec 21;27(1):1–8. doi: 10.1080/13510002.2021.2017681

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Cell apoptosis analysis of IPEC-J2 cells. (A) Annexin V/PI staining was used to detect cell apoptosis ratio. The green fluorescence of Annexin V-FITC represented by early apoptotic cells. The red fluorescence of PI staining indicated necrotic cells and apoptotic cells in the middle and late stages. The yellow color indicated that Annexin V-FITC and PI could stain the cells. (B) The expression level of apoptosis-related proteins was detected by western blot. (C) Effects of alfalfa saponins on MAPK pathway in IPEC-J2 cells. (D) Protective mechanism of alfalfa saponins in oxidative stress cells.