EtOH increases FoxO expression and downstream target catalase expression in MSCs. A, Relative FoxO3a mRNA expression in mesenchymal stem cells (MSCs) treated with 50 mM EtOH for 3, 6, and 9 hours. B, Western blot analysis of FoxO3a protein expression in MSCs treated with 50 mM EtOH at the same time points. Densitometry analysis is below Western blot. Hydrogen peroxide (H2O2) was used as a positive control. C, Relative catalase mRNA expression in MSCs treated with 50 mM EtOH for 3, 6, and 9 hours. Different letters demonstrate significance, n = 3, p < 0.05, 2-way ANOVA with Tukey’s post hoc test.