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. Author manuscript; available in PMC: 2022 Jan 4.
Published in final edited form as: Alcohol Clin Exp Res. 2020 May 18;44(6):1204–1213. doi: 10.1111/acer.14337

Fig. 3.

Fig. 3.

EtOH inhibits osteogenic and chondrogenic differentiation lineage markers. Relative osteogenic lineage marker RUNX2 (A) and Alp1 (B) mRNA expression in differentiating mesenchymal stem cells (MSCs) treated with 50 mM EtOH or media alone for 48 and 72 hours, respectively. Relative chondrogenic lineage marker SOX9 (C), COL2a1 (D), and aCaN (E) mRNA expression in differentiating MSCs treated with 50 mM EtOH or media alone for 48, 72, and 72 hours, respectively. Media control is shown to confirm differentiation marker expression and is not for statistical purposes. Different letters demonstrate significance, n = 3 to 5, p < 0.05, 2-tailed Student’s t-test.