Table 1.

DNA sequences of gRNA spacer used in this study.

gRNA name	Forward sequence (5′ to 3′)	Reverse sequence (5′ to 3′)
gRNA-1	GATTGCTCGCTAGCTATCGGTGTCA	AAACTGACACCGATAGCTAGCGAGC
gRNA-2	GATTGACTTCTCTGTGATGCCTTCT	AAACAGAAGGCATCACAGAGAAGTC
gRNA-3	GATTGTTGTAGTTGTAAATCCAAGA	AAACTCTTGGATTTACAACTACAAC
gRNA-4	GATTGTCCTCCTCCCAACTCCCAAG	AAACCTTGGGAGTTGGGAGGAGGAC
gRNA-5	GATTGAGGATGGGAGGTTGCCACTT	AAACAAGTGGCAACCTCCCATCCTC
gRNA-6	GATTGTAGGATGGGAGGTTGCCACT	AAACAGTGGCAACCTCCCATCCTAC
gRNA-7	GATTGACATCCCGCCCTCCTACACC	AAACGGTGTAGGAGGGCGGGATGTC
gRNA-8	GATTGTTGGAGAGGAGCCAGGTGT	AAACACACCTGGCTCCTCTCCAAC

Additional sequences for ligation with linearized vector by AarI are highlighted in bold.