Figure 4.
In vitro gene transfection efficiency was evaluated for PC9GR cells. (a,b) Images of PC9GR cells transfected with FITC-SCR siRNA detected by LSCM. Four groups were performed with different ultrasound intensity, namely the control group (the cells without any intervention), 0.26, 0.5, and 1.03 W/m2, and the irradiation time was 1 min (a). Additional four groups were performed to evaluate the effect of the duration, which was the control group (the cells without any intervention), 30 s, 1 min, and 3 min under the ultrasound intensity of 0.5 W/m2 (b). The cytoplasm of 9GR cells in the experimental group showed green fluorescence, but it was not detected in the nucleus. Blue (DAPI) represented the nucleus, while green (FITC) implied FITC-labeled siRNA. (c,d) Flow cytometry was used to detect the proportion of transfected cells in PC9GR cells under different ultrasound irradiation parameters. Four groups were performed with different ultrasound intensity, namely divided into four groups, namely, control group (the cells without any intervention), 0.26, 0.5, and 1.03 W/m2, and the irradiation time was 1 min (c). Additional four groups were performed to determine the effect of irradiation duration, which was the control group (the cells without any intervention), 30 s, 1 min, and 3 min under the ultrasound intensity of 0.5 W/m2 (d). (P3: The proportion of green fluorescent cells successfully transfected with FITC-labeled siRNA was detected by Flow cytometry).