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. 2021 Dec 21;12:762869. doi: 10.3389/fmicb.2021.762869

FIGURE 1.

FIGURE 1

EV-A71 2B inhibited type I interferon responses. HeLa cells were transfected with plasmids expressing HA-tagged 2B, followed by treatment with or without IFN-α (25 ng/μL). (A) EV-A71 2B inhibited the induction of ISGs induced by IFN-α. Total RNA was prepared from the cells for real-time RT-PCR to measure ISG mRNA transcripts. Values represent mean ± SD from triplicates of independent experiments.*P<0.05; **P<0.01. (B) EV-A71 2B inhibited the induction of MX1 and OAS1. The cell lysates were prepared for western blot analyses with antibodies for MX1 and OAS1. (C) EV-A71 2B prevented p-STAT1 from translocation into the nucleus upon IFN-α stimulation. The cells were fixed and premeabilized for subsequent staining with antibodies for HA tag and p-STAT1, which were subjected to confocal microscopy. Magnification, × 630. (D) Translocation of p-STAT1 and p-STAT2 was blocked by 2B. The cytosolic and nuclear fractions were prepared from the cell lysates, which were further subjected to western blot analyses with antibodies specific for p-STAT1 and p-STAT2.