FIGURE 7.

Expression and localization of viral 2B protein in EV-A71 infected cells. HeLa cells were infected with EV-A71 at an MOI of 1. Cell lysates were prepared at indicated time points p.i for western blot analyses with antibodies for VP1, 2B, KPNA1 or β-Tubulin (A). The cells, infected or non-infected, were fixed at indicated time points p.i. and subjected to confocal immunofluorescence imaging with antibodies for viral 2B (B). Cell nuclei were stained with DAPI and the mitochondria were stained with MitoTracker™ Deep Red FM. Magnification, × 630.